4I5V
Crystal structure of yeast Ap4A phosphorylase Apa2 in complex with Ap4A
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | SSRF BEAMLINE BL17U |
| Synchrotron site | SSRF |
| Beamline | BL17U |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2012-09-15 |
| Detector | ADSC QUANTUM 315r |
| Wavelength(s) | 0.97923 |
| Spacegroup name | C 1 2 1 |
| Unit cell lengths | 203.353, 52.781, 70.764 |
| Unit cell angles | 90.00, 99.92, 90.00 |
Refinement procedure
| Resolution | 42.070 - 2.696 |
| R-factor | 0.185 |
| Rwork | 0.181 |
| R-free | 0.26080 |
| Structure solution method | MOLECULAR REPLACEMENT |
| RMSD bond length | 0.008 |
| RMSD bond angle | 1.330 |
| Data reduction software | HKL-2000 |
| Data scaling software | HKL-2000 |
| Phasing software | MOLREP |
| Refinement software | PHENIX ((phenix.refine: 1.7.1_743)) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 50.000 | 2.790 |
| High resolution limit [Å] | 2.696 | 2.700 |
| Rmerge | 0.070 | 0.348 |
| Number of reflections | 19447 | |
| <I/σ(I)> | 7.4 | 1.9 |
| Completeness [%] | 93.9 | 96.7 |
| Redundancy | 2.2 | 2.2 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 8 | 289 | 23% PEG 2K, 0.1M Tris-HCl, pH 8.0, 20% glycerol, VAPOR DIFFUSION, HANGING DROP, temperature 289K |
