4I5U
Crystal structure of a fungal chimeric cellobiohydrolase Cel6A
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | SSRL BEAMLINE BL12-2 |
| Synchrotron site | SSRL |
| Beamline | BL12-2 |
| Detector technology | CCD |
| Detector | ADSC QUANTUM 315r |
| Wavelength(s) | 1.033 |
| Spacegroup name | C 1 2 1 |
| Unit cell lengths | 158.599, 45.414, 58.194 |
| Unit cell angles | 90.00, 95.47, 90.00 |
Refinement procedure
| Resolution | 35.308 - 1.220 |
| R-factor | 0.1434 |
| Rwork | 0.143 |
| R-free | 0.15840 |
| Structure solution method | MOLECULAR REPLACEMENT |
| RMSD bond length | 0.030 |
| RMSD bond angle | 2.614 |
| Data scaling software | SCALA (3.3.16) |
| Phasing software | MOLREP |
| Refinement software | REFMAC |
Data quality characteristics
| Overall | Inner shell | Outer shell | |
| Low resolution limit [Å] | 35.308 | 35.308 | 1.290 |
| High resolution limit [Å] | 1.220 | 3.860 | 1.220 |
| Rmerge | 0.017 | 0.215 | |
| Total number of observations | 7344 | 24424 | |
| Number of reflections | 106851 | ||
| <I/σ(I)> | 16.3 | 33.1 | 3.6 |
| Completeness [%] | 87.3 | 87.7 | 71.2 |
| Redundancy | 2.1 | 2.1 | 1.9 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, SITTING DROP | 6 | pH 6.0, VAPOR DIFFUSION, SITTING DROP |
