4I4K
Streptomyces globisporus C-1027 9-membered enediyne conserved protein SgcE6
Experimental procedure
| Experimental method | SAD |
| Source type | SYNCHROTRON |
| Source details | APS BEAMLINE 19-ID |
| Synchrotron site | APS |
| Beamline | 19-ID |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2012-04-15 |
| Detector | ADSC QUANTUM 315r |
| Wavelength(s) | 0.97921 |
| Spacegroup name | C 1 2 1 |
| Unit cell lengths | 72.715, 86.901, 55.265 |
| Unit cell angles | 90.00, 121.58, 90.00 |
Refinement procedure
| Resolution | 27.683 - 1.700 |
| R-factor | 0.169 |
| Rwork | 0.168 |
| R-free | 0.19500 |
| Structure solution method | SAD |
| RMSD bond length | 0.010 |
| RMSD bond angle | 1.325 |
| Data reduction software | HKL-3000 |
| Data scaling software | HKL-3000 |
| Phasing software | HKL-3000 |
| Refinement software | PHENIX ((phenix.refine: 1.8.1_1161)) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 50.000 | 1.730 |
| High resolution limit [Å] | 1.700 | 1.700 |
| Number of reflections | 31922 | |
| <I/σ(I)> | 11.9 | 3 |
| Completeness [%] | 99.3 | 98.2 |
| Redundancy | 3.9 | 2.8 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, SITTING DROP | 4.2 | 289 | 0.1 M disodium hydrogen phosphate citric acid pH 4.2, 40 %(w/v) PEG300, VAPOR DIFFUSION, SITTING DROP, temperature 289K |
