4I45
Crystal Structure of Orf6 protein from Photobacterium profundum, Mg2+-bound form
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | ALS BEAMLINE 4.2.2 |
| Synchrotron site | ALS |
| Beamline | 4.2.2 |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2009-10-25 |
| Detector | ADSC QUANTUM 315 |
| Wavelength(s) | 1.000 |
| Spacegroup name | I 2 2 2 |
| Unit cell lengths | 51.292, 54.362, 88.363 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 46.300 - 1.400 |
| R-factor | 0.15052 |
| Rwork | 0.148 |
| R-free | 0.19606 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 3r87 |
| RMSD bond length | 0.015 |
| RMSD bond angle | 1.608 |
| Data reduction software | d*TREK |
| Data scaling software | d*TREK |
| Phasing software | PHASER |
| Refinement software | REFMAC (5.6.0111) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 46.300 | 1.450 |
| High resolution limit [Å] | 1.400 | 1.400 |
| Number of reflections | 23821 | |
| Completeness [%] | 96.3 | 76.8 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 7.5 | 298 | 0.05 M magnesium chloride hexahydrate, 0.1 M HEPES pH 7.5, 30% v/v Polyethylene glycol monomethyl ether 550, VAPOR DIFFUSION, HANGING DROP, temperature 298K |
