4I3Z

Structure of pCDK2/CyclinA bound to ADP and 2 Magnesium ions

Experimental procedure

Source type	SYNCHROTRON
Source details	APS BEAMLINE 21-ID-F
Synchrotron site	APS
Beamline	21-ID-F
Temperature [K]	100
Detector technology	CCD
Collection date	2010-03-22
Detector	MARMOSAIC 225 mm CCD
Wavelength(s)	0.97872
Spacegroup name	P 1 21 1
Unit cell lengths	70.770, 164.130, 73.280
Unit cell angles	90.00, 107.07, 90.00

Refinement procedure

Resolution	42.790 - 2.050
R-factor	0.1989
Rwork	0.197
R-free	0.22780
Structure solution method	MOLECULAR REPLACEMENT
RMSD bond length	0.008
RMSD bond angle	0.887
Data reduction software	MOSFLM
Data scaling software	SCALA
Phasing software	PHASER
Refinement software	PHENIX ((phenix.refine: 1.7_650))

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	43.120	2.160
High resolution limit [Å]	2.050	2.050
Number of reflections	98449
<I/σ(I)>	7.5	1.6
Completeness [%]	98.6	97.3

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, SITTING DROP	7.5	293.15	22% w/v polyacrylic acid sodium salt 5100, 20mM MgCl2, 100mM HEPES pH 7.5, VAPOR DIFFUSION, SITTING DROP, temperature 293.15K