4I35

The crystal structure of serralysin

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	SSRF BEAMLINE BL17U
Synchrotron site	SSRF
Beamline	BL17U
Temperature [K]	100
Detector technology	CCD
Detector	ADSC QUANTUM 315r
Wavelength(s)	0.9799
Spacegroup name	P 21 21 21
Unit cell lengths	42.357, 105.075, 151.114
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	37.779 - 1.501
R-factor	0.1491
Rwork	0.148
R-free	0.16500
Structure solution method	MOLECULAR REPLACEMENT
RMSD bond length	0.019
RMSD bond angle	1.202
Data reduction software	HKL-2000
Data scaling software	HKL-2000
Phasing software	PHASER
Refinement software	PHENIX ((phenix.refine: 1.8.1_1168))

Data quality characteristics

	Overall
Low resolution limit [Å]	37.779
High resolution limit [Å]	1.501
Number of reflections	108473
Completeness [%]	99.9

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	EVAPORATION	6.3	295	30% peg8000, 0.1M ammonium sulfate, 0.1M citrate, pH 6.3, EVAPORATION, temperature 295K