4I34

Crystal Structure of W-W-W ClpX Hexamer

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	APS BEAMLINE 24-ID-C
Synchrotron site	APS
Beamline	24-ID-C
Temperature [K]	100
Detector technology	CCD
Collection date	2009-02-01
Detector	ADSC QUANTUM 315
Wavelength(s)	0.979
Spacegroup name	P 21 21 21
Unit cell lengths	58.341, 199.606, 203.441
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	38.337 - 4.122
R-factor	0.2761
Rwork	0.274
R-free	0.30770
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	3hws
RMSD bond length	0.003
RMSD bond angle	0.499
Data reduction software	DENZO
Data scaling software	SCALEPACK
Phasing software	PHASER
Refinement software	PHENIX (1.5_2)

Data quality characteristics

	Overall	Inner shell	Outer shell
Low resolution limit [Å]	50.000	50.000	4.300
High resolution limit [Å]	4.122	8.930	4.122
Rmerge	0.059	0.034	0.294
Number of reflections	18530
<I/σ(I)>	11.1
Completeness [%]	97.2	98.2	84.1
Redundancy	5.7	5.6	4.3

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION	4.8	298	75 mM sodium acetate, 1.9 M ammonium sulfate, pH 4.8, VAPOR DIFFUSION, temperature 298K