4I2W

Crystal structure of the myosin chaperone UNC-45 from C.elegans in complex with a Hsp70 peptide

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	SLS BEAMLINE X06DA
Synchrotron site	SLS
Beamline	X06DA
Temperature [K]	100
Detector technology	PIXEL
Collection date	2012-03-08
Detector	DECTRIS PILATUS 2M
Wavelength(s)	0.979
Spacegroup name	P 61 2 2
Unit cell lengths	86.276, 86.276, 808.978
Unit cell angles	90.00, 90.00, 120.00

Refinement procedure

Resolution	47.817 - 3.600
Rwork	0.234
R-free	0.28450
Structure solution method	MOLECULAR REPLACEMENT
Data scaling software	SCALA (3.3.16)
Refinement software	CNS

Data quality characteristics

	Overall	Inner shell	Outer shell
Low resolution limit [Å]	50.000	47.817	3.790
High resolution limit [Å]	3.600	11.380	3.600
Rmerge		0.053	0.697
Total number of observations		4280	18942
Number of reflections	22485
<I/σ(I)>	8.5	11.6	1.1
Completeness [%]	99.9	98.3	100
Redundancy	6.1	4.7	6

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, SITTING DROP	7	292	0.1 M HEPES, 10% PEG 8000, 12% ethylene glycol, pH 7.0, VAPOR DIFFUSION, SITTING DROP, temperature 292K