4I2O

The Structure of FixK2 from Bradyrhizobium japonicum

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	SLS BEAMLINE X06SA
Synchrotron site	SLS
Beamline	X06SA
Temperature [K]	90
Detector technology	PIXEL
Collection date	2011-09-13
Detector	PSI PILATUS 6M
Wavelength(s)	0.997
Spacegroup name	P 1 21 1
Unit cell lengths	111.330, 43.880, 70.110
Unit cell angles	90.00, 90.04, 90.00

Refinement procedure

Resolution	43.579 - 1.770
R-factor	0.1852
Rwork	0.183
R-free	0.22710
Structure solution method	SAD
RMSD bond length	0.018
RMSD bond angle	2.064
Data reduction software	XDS (package)
Data scaling software	XDS (package)
Phasing software	SHELXS
Refinement software	PHENIX ((phenix.refine: 1.7.3_928))

Data quality characteristics

	Overall
Low resolution limit [Å]	43.580
High resolution limit [Å]	1.770
Number of reflections	66510
Completeness [%]	99.7

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, SITTING DROP	6	277	0.1M BisTris, 0.1M NH4-acetate, 16-22% PEG 10000, VAPOR DIFFUSION, SITTING DROP, temperature 277K
1	VAPOR DIFFUSION, SITTING DROP	6	277	0.1M BisTris, 0.1M NH4-acetate, 16-22% PEG 10000, VAPOR DIFFUSION, SITTING DROP, temperature 277K