4I21
Crystal structure of L858R + T790M EGFR kinase domain in complex with MIG6 peptide
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | APS BEAMLINE 17-ID |
| Synchrotron site | APS |
| Beamline | 17-ID |
| Temperature [K] | 98 |
| Detector technology | PIXEL |
| Collection date | 2011-06-09 |
| Detector | PSI PILATUS 6M |
| Wavelength(s) | 1.0 |
| Spacegroup name | P 1 |
| Unit cell lengths | 38.666, 66.050, 94.518 |
| Unit cell angles | 71.85, 87.38, 74.26 |
Refinement procedure
| Resolution | 37.180 - 3.370 |
| R-factor | 0.233 |
| Rwork | 0.230 |
| R-free | 0.27900 |
| Structure solution method | MOLECULAR REPLACEMENT |
| RMSD bond length | 0.008 |
| RMSD bond angle | 1.100 |
| Data scaling software | autoPROC |
| Phasing software | CNX |
| Refinement software | CNX |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 42.990 | 3.560 |
| High resolution limit [Å] | 3.370 | 3.370 |
| Rmerge | 0.085 | 0.399 |
| Number of reflections | 11744 | |
| <I/σ(I)> | 10 | 2.6 |
| Completeness [%] | 97.4 | 96.9 |
| Redundancy | 3.5 | 3.5 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 294 | 14.5% PEG 8,000, 0.1M Na Acetate trihydrate pH 4.8-5.5, 0.2-0.38 M K acetate, VAPOR DIFFUSION, HANGING DROP, temperature 294K |
