4I1G
dna octamer d(GTseGTACAC) partially crosslinked with two platinums
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | ALS BEAMLINE 12.3.1 |
Synchrotron site | ALS |
Beamline | 12.3.1 |
Temperature [K] | 200 |
Detector technology | CCD |
Collection date | 2012-09-27 |
Detector | CUSTOM-MADE |
Wavelength(s) | 0.97 |
Spacegroup name | P 43 21 2 |
Unit cell lengths | 42.368, 42.368, 24.056 |
Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
Resolution | 15.900 - 1.250 |
R-factor | 0.20947 |
Rwork | 0.209 |
R-free | 0.21729 |
Structure solution method | MOLECULAR REPLACEMENT |
Starting model (for MR) | 1dns |
RMSD bond length | 0.040 |
RMSD bond angle | 3.373 |
Data reduction software | HKL-2000 |
Data scaling software | HKL-2000 |
Phasing software | PHASES |
Refinement software | REFMAC (5.5.0110) |
Data quality characteristics
Overall | |
Low resolution limit [Å] | 15.900 |
High resolution limit [Å] | 1.250 |
Rmerge | 0.094 |
Number of reflections | 5900 |
<I/σ(I)> | 25.6 |
Completeness [%] | 94.3 |
Redundancy | 11.7 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, HANGING DROP | 6 | 298 | 10% MPD, 40 mM Sodium codylate trihydrate, 12 mM Spermine tetrahydrochloride, 80 mM Strontium chloride , pH 6.0, VAPOR DIFFUSION, HANGING DROP, temperature 298.0K |