4I0B
structure of the mutant Catabolite gene activator protein H160L
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | DIAMOND BEAMLINE I04 |
Synchrotron site | Diamond |
Beamline | I04 |
Temperature [K] | 100 |
Detector technology | CCD |
Collection date | 2011-10-29 |
Detector | ADSC QUANTUM 315 |
Wavelength(s) | 0.9687 |
Spacegroup name | P 1 21 1 |
Unit cell lengths | 56.780, 72.320, 58.410 |
Unit cell angles | 90.00, 109.89, 90.00 |
Refinement procedure
Resolution | 54.900 - 1.500 |
R-factor | 0.18172 |
Rwork | 0.180 |
R-free | 0.20833 |
Structure solution method | MOLECULAR REPLACEMENT |
RMSD bond length | 0.011 |
RMSD bond angle | 1.601 |
Data reduction software | MOSFLM |
Data scaling software | SCALA |
Phasing software | PHASER |
Refinement software | REFMAC (5.6.0117) |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 54.900 | 1.539 |
High resolution limit [Å] | 1.500 | 1.500 |
Number of reflections | 67230 | |
Completeness [%] | 99.7 | 99.6 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, HANGING DROP | 6.5 | 293 | 7-10% Peg3350, 15-20% MPD, pH 6.5, VAPOR DIFFUSION, HANGING DROP, temperature 293K |