4HZC
Crystal structure of Serine acetyltransferase from Brucella abortus strain S19
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | ESRF BEAMLINE BM14 |
| Synchrotron site | ESRF |
| Beamline | BM14 |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2011-12-03 |
| Wavelength(s) | 0.97 |
| Spacegroup name | P 1 21 1 |
| Unit cell lengths | 73.697, 256.774, 82.285 |
| Unit cell angles | 90.00, 91.20, 90.00 |
Refinement procedure
| Resolution | 50.000 - 1.970 |
| R-factor | 0.18944 |
| Rwork | 0.187 |
| R-free | 0.23899 |
| Structure solution method | MOLECULAR REPLACEMENT |
| RMSD bond length | 0.016 |
| RMSD bond angle | 1.606 |
| Data reduction software | DENZO |
| Data scaling software | SCALEPACK |
| Phasing software | PHASER (2.1.4) |
| Refinement software | REFMAC |
Data quality characteristics
| Overall | Inner shell | Outer shell | |
| Low resolution limit [Å] | 128.390 | 50.000 | 1.990 |
| High resolution limit [Å] | 1.960 | 5.320 | 1.960 |
| Rmerge | 0.038 | 0.022 | 0.328 |
| Number of reflections | 184406 | ||
| <I/σ(I)> | 19.8 | ||
| Completeness [%] | 86.0 | 97.2 | 83 |
| Redundancy | 2.4 | 3.1 | 2.2 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 7.4 | 289 | 7% PEG 1000, 7% PEG 3350, 5% MPD, 0.12 M Ethylene glycol, 100 mM Tris, pH 7.4, VAPOR DIFFUSION, HANGING DROP, temperature 289K |
