4HZC
Crystal structure of Serine acetyltransferase from Brucella abortus strain S19
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | ESRF BEAMLINE BM14 |
Synchrotron site | ESRF |
Beamline | BM14 |
Temperature [K] | 100 |
Detector technology | CCD |
Collection date | 2011-12-03 |
Wavelength(s) | 0.97 |
Spacegroup name | P 1 21 1 |
Unit cell lengths | 73.697, 256.774, 82.285 |
Unit cell angles | 90.00, 91.20, 90.00 |
Refinement procedure
Resolution | 50.000 - 1.970 |
R-factor | 0.18944 |
Rwork | 0.187 |
R-free | 0.23899 |
Structure solution method | MOLECULAR REPLACEMENT |
RMSD bond length | 0.016 |
RMSD bond angle | 1.606 |
Data reduction software | DENZO |
Data scaling software | SCALEPACK |
Phasing software | PHASER (2.1.4) |
Refinement software | REFMAC |
Data quality characteristics
Overall | Inner shell | Outer shell | |
Low resolution limit [Å] | 128.390 | 50.000 | 1.990 |
High resolution limit [Å] | 1.960 | 5.320 | 1.960 |
Rmerge | 0.038 | 0.022 | 0.328 |
Number of reflections | 184406 | ||
<I/σ(I)> | 19.8 | ||
Completeness [%] | 86.0 | 97.2 | 83 |
Redundancy | 2.4 | 3.1 | 2.2 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, HANGING DROP | 7.4 | 289 | 7% PEG 1000, 7% PEG 3350, 5% MPD, 0.12 M Ethylene glycol, 100 mM Tris, pH 7.4, VAPOR DIFFUSION, HANGING DROP, temperature 289K |