4HZ3
MthK pore crystallized in presence of TBSb
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | NSLS BEAMLINE X25 |
| Synchrotron site | NSLS |
| Beamline | X25 |
| Temperature [K] | 93 |
| Detector technology | CCD |
| Collection date | 2011-12-14 |
| Detector | ADSC QUANTUM 315 |
| Wavelength(s) | 1.1 |
| Spacegroup name | P 1 |
| Unit cell lengths | 43.965, 63.396, 63.311 |
| Unit cell angles | 89.99, 89.95, 90.00 |
Refinement procedure
| Resolution | 36.128 - 1.700 |
| R-factor | 0.1736 |
| Rwork | 0.173 |
| R-free | 0.19160 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 3ldc |
| RMSD bond length | 1.100 |
| RMSD bond angle | 0.009 |
| Data reduction software | HKL-2000 |
| Data scaling software | SCALEPACK |
| Phasing software | PHASER (2.4.0) |
| Refinement software | PHENIX (1.7.3_928) |
Data quality characteristics
| Overall | Inner shell | Outer shell | |
| Low resolution limit [Å] | 50.000 | 50.000 | 1.760 |
| High resolution limit [Å] | 1.700 | 3.660 | 1.700 |
| Rmerge | 0.048 | 0.028 | 0.677 |
| Number of reflections | 73276 | ||
| <I/σ(I)> | 13.1 | ||
| Completeness [%] | 96.4 | 99 | 93.2 |
| Redundancy | 3.3 | 3.3 | 3.1 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, SITTING DROP | 6.5 | 293 | 3.5 4.0M 1,6-hexanediol, 100mM MES, 10mM TBSb , pH 6.5, VAPOR DIFFUSION, SITTING DROP, temperature 293K |
