4HYW
Pyruvate kinase (PYK) from Trypanosoma brucei in the presence of Magnesium and F26BP
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | DIAMOND BEAMLINE I24 |
Synchrotron site | Diamond |
Beamline | I24 |
Temperature [K] | 100 |
Detector technology | PIXEL |
Collection date | 2011-07-22 |
Detector | DECTRIS PILATUS 6M |
Wavelength(s) | 0.96860 |
Spacegroup name | I 2 2 2 |
Unit cell lengths | 103.680, 109.000, 268.380 |
Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
Resolution | 54.500 - 2.350 |
R-factor | 0.16566 |
Rwork | 0.164 |
R-free | 0.19873 |
Structure solution method | MOLECULAR REPLACEMENT |
RMSD bond length | 0.010 |
RMSD bond angle | 0.986 |
Data reduction software | MOSFLM |
Data scaling software | SCALA |
Phasing software | PHASER |
Refinement software | REFMAC (5.6.0117) |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 134.190 | 2.480 |
High resolution limit [Å] | 2.350 | 2.350 |
Number of reflections | 63644 | |
Completeness [%] | 100.0 | 100 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, HANGING DROP | 7.2 | 277 | 6-20% PEG 8,000, 10-20% glycerol, 50 mM TEA buffer, 100 mM KCl, 50 mM MgCl2, 80 uM F26BP, 800 uM Ponceau S., pH 7.2, VAPOR DIFFUSION, HANGING DROP, temperature 277K |