4HYW

Pyruvate kinase (PYK) from Trypanosoma brucei in the presence of Magnesium and F26BP

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	DIAMOND BEAMLINE I24
Synchrotron site	Diamond
Beamline	I24
Temperature [K]	100
Detector technology	PIXEL
Collection date	2011-07-22
Detector	DECTRIS PILATUS 6M
Wavelength(s)	0.96860
Spacegroup name	I 2 2 2
Unit cell lengths	103.680, 109.000, 268.380
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	54.500 - 2.350
R-factor	0.16566
Rwork	0.164
R-free	0.19873
Structure solution method	MOLECULAR REPLACEMENT
RMSD bond length	0.010
RMSD bond angle	0.986
Data reduction software	MOSFLM
Data scaling software	SCALA
Phasing software	PHASER
Refinement software	REFMAC (5.6.0117)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	134.190	2.480
High resolution limit [Å]	2.350	2.350
Number of reflections	63644
Completeness [%]	100.0	100

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, HANGING DROP	7.2	277	6-20% PEG 8,000, 10-20% glycerol, 50 mM TEA buffer, 100 mM KCl, 50 mM MgCl2, 80 uM F26BP, 800 uM Ponceau S., pH 7.2, VAPOR DIFFUSION, HANGING DROP, temperature 277K