4HYQ

Crystal structure of phospholipase A1 from Streptomyces albidoflavus NA297

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	PHOTON FACTORY BEAMLINE BL-1A
Synchrotron site	Photon Factory
Beamline	BL-1A
Temperature [K]	100
Detector technology	CCD
Collection date	2011-03-05
Detector	ADSC QUANTUM 270
Wavelength(s)	1.0
Spacegroup name	P 31 2 1
Unit cell lengths	67.550, 67.550, 96.112
Unit cell angles	90.00, 90.00, 120.00

Refinement procedure

Resolution	37.100 - 1.750
R-factor	0.186
Rwork	0.186
R-free	0.22300
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	1esc
RMSD bond length	0.009
RMSD bond angle	1.500
Data reduction software	HKL-2000
Data scaling software	HKL-2000
Phasing software	MOLREP
Refinement software	CNS (1.1)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	37.100	1.810
High resolution limit [Å]	1.750	1.750
Number of reflections	26008
<I/σ(I)>	29.5	5.8
Completeness [%]	100.0	100
Redundancy	10.9

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, HANGING DROP	7.5	293	1.8M ammonium sulfate, 3% PEGMME2000, 0.1M Hepes, pH 7.5, VAPOR DIFFUSION, HANGING DROP, temperature 293K