4HXJ
Crystal structure of SH3:RGT complex
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | SSRF BEAMLINE BL17U |
| Synchrotron site | SSRF |
| Beamline | BL17U |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2010-04-30 |
| Detector | ADSC QUANTUM 315r |
| Wavelength(s) | 0.97885 |
| Spacegroup name | P 1 21 1 |
| Unit cell lengths | 27.980, 40.830, 43.340 |
| Unit cell angles | 90.00, 105.97, 90.00 |
Refinement procedure
| Resolution | 29.163 - 2.000 |
| R-factor | 0.1445 |
| Rwork | 0.139 |
| R-free | 0.19250 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 1fmk |
| RMSD bond length | 0.007 |
| RMSD bond angle | 1.025 |
| Data reduction software | MOSFLM |
| Data scaling software | SCALA |
| Phasing software | PHASER |
| Refinement software | PHENIX ((phenix.refine)) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 29.200 | 2.080 |
| High resolution limit [Å] | 2.000 | 2.000 |
| Rmerge | 0.170 | 0.360 |
| Number of reflections | 6468 | |
| <I/σ(I)> | 10 | 7 |
| Completeness [%] | 99.0 | 100 |
| Redundancy | 8 | 8 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 8 | 298 | 100mM Tris, pH 8.0, 1.3M sodium citrate, VAPOR DIFFUSION, HANGING DROP, temperature 298K |
