4HXG

Pyrococcus horikoshii acylaminoacyl peptidase (orthorhombic crystal form)

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	ESRF BEAMLINE BM14
Synchrotron site	ESRF
Beamline	BM14
Temperature [K]	100
Detector technology	CCD
Collection date	2006-06-29
Detector	MARMOSAIC 225 mm CCD
Wavelength(s)	1.0715
Spacegroup name	P 21 21 21
Unit cell lengths	183.310, 183.800, 275.740
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	19.980 - 2.700
R-factor	0.2002
Rwork	0.200
R-free	0.24070
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	4hxf
RMSD bond length	0.005
RMSD bond angle	0.979
Data reduction software	XDS
Data scaling software	XSCALE
Phasing software	MOLREP
Refinement software	PHENIX ((phenix.refine: 1.7_650))

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	20.000	2.720
High resolution limit [Å]	2.650	2.650
Rmerge	0.090	0.690
Number of reflections	255208
<I/σ(I)>	16.47	2.36
Completeness [%]	94.9	96.8
Redundancy	4.39	4.335

Crystallization Conditions

crystal ID	method	pH	temperature	details
1