4HXC

Crystal structure of a putative glycosyl hydrolase (BACUNI_00951) from Bacteroides uniformis ATCC 8492 at 2.15 A resolution

Experimental procedure

Experimental method	MAD
Source type	SYNCHROTRON
Source details	SSRL BEAMLINE BL11-1
Synchrotron site	SSRL
Beamline	BL11-1
Temperature [K]	100
Detector technology	PIXEL
Collection date	2012-06-28
Detector	DECTRIS PILATUS 6M
Wavelength(s)	0.91837,0.9792,0.97868
Spacegroup name	P 43 21 2
Unit cell lengths	91.537, 91.537, 99.260
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	29.461 - 2.150
R-factor	0.1857
Rwork	0.184
R-free	0.22210
Structure solution method	MAD
RMSD bond length	0.010
RMSD bond angle	1.080
Data reduction software	XDS
Data scaling software	XSCALE (March 15, 2012)
Phasing software	SHELX
Refinement software	BUSTER-TNT (2.10.0)

Data quality characteristics

	Overall	Inner shell	Outer shell
Low resolution limit [Å]	29.461		2.230
High resolution limit [Å]	2.150	4.610	2.150
Rmerge	0.073	0.027	0.421
Number of reflections	22690	4236	4010
<I/σ(I)>	9.33	23.6	2.1
Completeness [%]	93.0	94.4	87.7

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, SITTING DROP		277	0.2M potassium fluoride 20% polyethylene glycol 3350, NANODROP, VAPOR DIFFUSION, SITTING DROP, temperature 277K