4HX6
Streptomyces globisporus C-1027 NADH:FAD oxidoreductase SgcE6
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | APS BEAMLINE 19-ID |
| Synchrotron site | APS |
| Beamline | 19-ID |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2012-04-16 |
| Detector | ADSC QUANTUM 315 |
| Wavelength(s) | 0.97912 |
| Spacegroup name | P 1 21 1 |
| Unit cell lengths | 56.374, 213.307, 56.716 |
| Unit cell angles | 90.00, 88.83, 90.00 |
Refinement procedure
| Resolution | 30.121 - 1.890 |
| R-factor | 0.1689 |
| Rwork | 0.167 |
| R-free | 0.21190 |
| Structure solution method | SAD |
| RMSD bond length | 0.007 |
| RMSD bond angle | 1.083 |
| Data reduction software | HKL-3000 |
| Data scaling software | HKL-3000 |
| Phasing software | SHELXD |
| Refinement software | PHENIX ((phenix.refine: 1.7.1_743)) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 30.200 | 1.930 |
| High resolution limit [Å] | 1.890 | 1.900 |
| Rmerge | 0.095 | 0.452 |
| Number of reflections | 104829 | |
| <I/σ(I)> | 19.5 | 2.4 |
| Completeness [%] | 98.3 | 80.9 |
| Redundancy | 3.3 | 2.8 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, SITTING DROP | 7.5 | 289 | 0.2M Ammonium sulfate, 0.1M HEPES:NaOH, 25% (w/v) PEG3350, pH 7.5, VAPOR DIFFUSION, SITTING DROP, temperature 289K |
