4HX1
Structure of HLA-A68 complexed with a tumor antigen derived peptide
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | SSRF BEAMLINE BL17U |
| Synchrotron site | SSRF |
| Beamline | BL17U |
| Detector technology | IMAGE PLATE |
| Collection date | 2012-05-01 |
| Detector | RIGAKU RAXIS IV++ |
| Wavelength(s) | 0.97930 |
| Spacegroup name | C 1 2 1 |
| Unit cell lengths | 129.237, 37.857, 108.323 |
| Unit cell angles | 90.00, 120.04, 90.00 |
Refinement procedure
| Resolution | 31.461 - 1.802 |
| R-factor | 0.1918 |
| Rwork | 0.190 |
| R-free | 0.22610 |
| Structure solution method | MOLECULAR REPLACEMENT |
| RMSD bond length | 0.005 |
| RMSD bond angle | 0.958 |
| Data reduction software | HKL-2000 |
| Data scaling software | HKL-2000 |
| Phasing software | CNS |
| Refinement software | CNS |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 50.000 | 1.860 |
| High resolution limit [Å] | 1.800 | 1.800 |
| Rmerge | 0.271 | |
| Number of reflections | 41016 | |
| <I/σ(I)> | 4.906 | |
| Completeness [%] | 95.4 | 99.9 |
| Redundancy | 4 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 8.5 | 291 | 100mM Tris, 8% PEG8000, pH 8.5, VAPOR DIFFUSION, HANGING DROP, temperature 291K |
