4HWW
Crystal structure of human Arginase-1 complexed with inhibitor 9
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | SLS BEAMLINE X06DA |
| Synchrotron site | SLS |
| Beamline | X06DA |
| Temperature [K] | 100 |
| Detector technology | PIXEL |
| Collection date | 2012-04-02 |
| Detector | DECTRIS PILATUS 6M |
| Spacegroup name | P 3 |
| Unit cell lengths | 90.143, 90.143, 69.328 |
| Unit cell angles | 90.00, 90.00, 120.00 |
Refinement procedure
| Resolution | 25.919 - 1.298 |
| R-factor | 0.1585 |
| Rwork | 0.151 |
| R-free | 0.16860 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 1d3v |
| RMSD bond length | 0.007 |
| RMSD bond angle | 1.274 |
| Data reduction software | DENZO |
| Data scaling software | SCALEPACK |
| Phasing software | AMoRE |
| Refinement software | PHENIX (1.8_1069) |
Data quality characteristics
| Overall | Inner shell | Outer shell | |
| Low resolution limit [Å] | 50.000 | 50.000 | 1.350 |
| High resolution limit [Å] | 1.298 | 2.800 | 1.300 |
| Rmerge | 0.051 | 0.024 | 0.591 |
| Number of reflections | 155548 | ||
| <I/σ(I)> | 12.5 | ||
| Completeness [%] | 99.9 | 99.4 | 99.6 |
| Redundancy | 4.9 | 4.9 | 4.3 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 5 | 277 | 100 mM Malonic Acid, Imidazol, Boric system, 25% Polyethylene Glycol (PEG) 1500, vapor diffusion, hanging drop, temperature 277K |
