4HWL
Crystal Structure Analysis of the Bacteriorhodopsin in Facial Amphiphile-7 DMPC Bicelle
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | SSRL BEAMLINE BL11-1 |
| Synchrotron site | SSRL |
| Beamline | BL11-1 |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2011-04-15 |
| Detector | ADSC QUANTUM 315 |
| Wavelength(s) | 0.97945 |
| Spacegroup name | P 1 21 1 |
| Unit cell lengths | 46.160, 113.290, 56.190 |
| Unit cell angles | 90.00, 114.27, 90.00 |
Refinement procedure
| Resolution | 42.080 - 2.000 |
| R-factor | 0.15942 |
| Rwork | 0.159 |
| R-free | 0.17183 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 1kme |
| RMSD bond length | 0.021 |
| RMSD bond angle | 1.879 |
| Data reduction software | MOSFLM |
| Data scaling software | SCALA |
| Phasing software | PHASES |
| Refinement software | REFMAC (5.7.0029) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 46.670 | 2.110 |
| High resolution limit [Å] | 2.000 | 2.000 |
| Rmerge | 0.074 | 0.550 |
| Number of reflections | 34192 | |
| <I/σ(I)> | 6.5 | 1.7 |
| Completeness [%] | 96.6 | 95.1 |
| Redundancy | 2.1 | 2.1 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, SITTING DROP | 3.4 | 300.15 | 3.6M Sodium Phosphate (pH 3.4), 3.5% Triethyleneglycol, 180mM Hexanediol, VAPOR DIFFUSION, SITTING DROP, temperature 300.15K |
