4HVY

A thermostable variant of human NUDT18 NUDIX domain obtained by Hot Colony Filtration

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	NSRRC BEAMLINE BL13B1
Synchrotron site	NSRRC
Beamline	BL13B1
Temperature [K]	100
Detector technology	CCD
Collection date	2010-06-03
Detector	ADSC QUANTUM 315r
Wavelength(s)	1.0
Spacegroup name	I 41 2 2
Unit cell lengths	90.200, 90.200, 75.030
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	28.524 - 1.460
R-factor	0.1685
Rwork	0.166
R-free	0.21050
Structure solution method	MOLECULAR REPLACEMENT
RMSD bond length	0.007
RMSD bond angle	1.147
Data reduction software	MOSFLM
Data scaling software	SCALA
Phasing software	PHASER
Refinement software	PHENIX ((phenix.refine: 1.8_1069))

Data quality characteristics

	Overall	Inner shell	Outer shell
Low resolution limit [Å]	32.340	32.340	1.540
High resolution limit [Å]	1.400	4.620	1.400
Number of reflections	26714
Completeness [%]	98.7	68.25	50.59

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, SITTING DROP	5	293	0.1M tri-sodium citrate, 3.2M ammonium sulfate, pH 5.0, VAPOR DIFFUSION, SITTING DROP, temperature 293K