4HVF

Crystal structure of green fluorescent protein lanGFP(Branchiostoma Lanceolatum)

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	APS BEAMLINE 22-ID
Synchrotron site	APS
Beamline	22-ID
Temperature [K]	100
Detector technology	CCD
Detector	MARMOSAIC 300 mm CCD
Wavelength(s)	1.0
Spacegroup name	P 21 21 21
Unit cell lengths	72.187, 105.802, 121.130
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	26.560 - 1.700
R-factor	0.177
Rwork	0.177
R-free	0.21340
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	2g3o
RMSD bond length	0.030
RMSD bond angle	2.127
Data scaling software	SCALEPACK
Refinement software	REFMAC

Data quality characteristics

	Overall	Inner shell	Outer shell
Low resolution limit [Å]	50.000	50.000	1.770
High resolution limit [Å]	1.700	3.680	1.700
Rmerge	0.081	0.053	0.415
Number of reflections	101261
<I/σ(I)>	10.2
Completeness [%]	99.5	99.7	95.3
Redundancy	7.3	7.2	6.4

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, HANGING DROP	5.5	293	20 mM Tris pH 8.0, 200 mM NaCl, 5 mM EDTA mixed with an equal amount of reservoir solutions: 0.2M MgCl2x6H2O, 0.1M BIS-Tris pH 5.5, 25% PEG3350, VAPOR DIFFUSION, HANGING DROP, temperature 293.0K