4HUD

Structure of the bacteriophage T4 tail terminator protein, gp15.

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	APS BEAMLINE 14-BM-C
Synchrotron site	APS
Beamline	14-BM-C
Temperature [K]	100
Detector technology	CCD
Collection date	2001-08-23
Detector	ADSC QUANTUM 315
Wavelength(s)	1.0
Spacegroup name	P 32
Unit cell lengths	100.682, 100.682, 155.891
Unit cell angles	90.00, 90.00, 120.00

Refinement procedure

Resolution	32.956 - 2.700
R-factor	0.2092
Rwork	0.207
R-free	0.25210
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	4huh
RMSD bond length	0.010
RMSD bond angle	1.298
Data reduction software	HKL-2000
Data scaling software	HKL-2000
Phasing software	PHASER
Refinement software	PHENIX ((phenix.refine: 1.8.1_1168))

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	33.000	2.750
High resolution limit [Å]	2.700	2.700
Rmerge	0.078	0.270
Number of reflections	45176
<I/σ(I)>	20	3.3
Completeness [%]	93.0	85
Redundancy	5.5	2.3

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, HANGING DROP	7.5	301	0.15 M ammonium sulfate, 10.5 % isopropanol, 0.085 M Na Hepes pH 7.5, 20% PEG 4000, 15% glycerol., VAPOR DIFFUSION, HANGING DROP, temperature 301K