4HU8
Crystal Structure of a Bacterial Ig-like Domain Containing GH10 Xylanase from Termite Gut
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | NSLS BEAMLINE X29A |
| Synchrotron site | NSLS |
| Beamline | X29A |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2011-09-06 |
| Detector | ADSC QUANTUM 315r |
| Wavelength(s) | 1.075 |
| Spacegroup name | P 1 |
| Unit cell lengths | 55.685, 86.963, 246.433 |
| Unit cell angles | 92.96, 90.86, 108.56 |
Refinement procedure
| Resolution | 49.080 - 2.000 |
| R-factor | 0.22115 |
| Rwork | 0.219 |
| R-free | 0.26624 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 1nq6 |
| RMSD bond length | 0.012 |
| RMSD bond angle | 1.200 |
| Data reduction software | DENZO |
| Data scaling software | SCALEPACK |
| Phasing software | MOLREP |
| Refinement software | REFMAC (5.6.0117) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 50.000 | 2.070 |
| High resolution limit [Å] | 2.000 | 2.000 |
| Rmerge | 0.098 | 0.603 |
| Number of reflections | 293766 | |
| <I/σ(I)> | 15 | 2 |
| Completeness [%] | 97.7 | 96 |
| Redundancy | 4.1 | 3.7 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 5.6 | 280 | 0.2 M potassium sodium tartrate tetrahydrate, 0.1 M sodium citrate tribasic dihydrate, 2 M ammonium sulfate, 20% glycerol, pH 5.6, VAPOR DIFFUSION, HANGING DROP, temperature 280K |
