4HU0
Crystal Structure of a metagenome-derived cellulase Cel5A in complex with cellotetraose
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | PAL/PLS BEAMLINE 6C1 |
| Synchrotron site | PAL/PLS |
| Beamline | 6C1 |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2011-06-01 |
| Detector | ADSC QUANTUM 210 |
| Spacegroup name | P 21 3 |
| Unit cell lengths | 136.924, 136.924, 136.924 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 43.300 - 2.380 |
| R-factor | 0.154 |
| Rwork | 0.152 |
| R-free | 0.18800 |
| Structure solution method | MOLECULAR REPLACEMENT |
| RMSD bond length | 0.032 |
| RMSD bond angle | 2.528 |
| Data reduction software | DENZO |
| Data scaling software | SCALEPACK |
| Phasing software | AMoRE |
| Refinement software | REFMAC (5.5.0109) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 50.000 | 2.470 |
| High resolution limit [Å] | 2.380 | 2.380 |
| Number of reflections | 34430 | |
| Completeness [%] | 100.0 | 99.6 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | 6.5 | 291 | PEG 4000, MES, pH 6.5, VAPOR DIFFUSION, HANGING DROP, temperature 291K |
