4HTY
Crystal Structure of a metagenome-derived cellulase Cel5A
Experimental procedure
| Experimental method | MAD |
| Source type | SYNCHROTRON |
| Source details | NSRRC BEAMLINE BL13B1 |
| Synchrotron site | NSRRC |
| Beamline | BL13B1 |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2011-07-29 |
| Detector | ADSC QUANTUM 210 |
| Wavelength(s) | 0.97916, 0.97933, 0.96394 |
| Spacegroup name | P 21 3 |
| Unit cell lengths | 137.771, 137.771, 137.771 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 28.120 - 2.000 |
| R-factor | 0.155 |
| Rwork | 0.154 |
| R-free | 0.16900 |
| Structure solution method | MAD |
| RMSD bond length | 0.041 |
| RMSD bond angle | 2.798 |
| Data reduction software | HKL-2000 |
| Data scaling software | HKL-2000 |
| Phasing software | SOLVE |
| Refinement software | REFMAC (5.5.0109) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 30.000 | 2.070 |
| High resolution limit [Å] | 2.000 | 2.000 |
| Number of reflections | 59067 | |
| Completeness [%] | 99.9 | 100 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | 6.5 | 291 | PEG4000, MES pH 6.5, VAPOR DIFFUSION, HANGING DROP, temperature 291K |
