4HTS
Crystal Structure of Twin Arginine Translocase Receptor- TatC
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | SSRL BEAMLINE BL12-2 |
Synchrotron site | SSRL |
Beamline | BL12-2 |
Temperature [K] | 100 |
Detector technology | CCD |
Collection date | 2008-11-25 |
Detector | MARMOSAIC 325 mm CCD |
Wavelength(s) | 1.08 |
Spacegroup name | P 41 2 2 |
Unit cell lengths | 109.780, 109.780, 107.020 |
Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
Resolution | 29.911 - 4.000 |
R-factor | 0.2916 |
Rwork | 0.288 |
R-free | 0.32310 |
Structure solution method | MOLECULAR REPLACEMENT |
RMSD bond length | 0.003 |
RMSD bond angle | 0.882 |
Data reduction software | HKL-2000 |
Data scaling software | SCALA |
Phasing software | PHASER |
Refinement software | PHENIX (1.8.1_1168) |
Data quality characteristics
Overall | Inner shell | Outer shell | |
Low resolution limit [Å] | 110.430 | 39.040 | 4.320 |
High resolution limit [Å] | 4.000 | 12.970 | 4.100 |
Number of reflections | 5887 | ||
<I/σ(I)> | 16.8 | ||
Completeness [%] | 99.8 | 96.3 | 99.8 |
Redundancy | 7.7 | 8.1 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, SITTING DROP | 7.5 | 293 | 28% Jeffamine ED-2001 0.05M HEPES pH 7.5 10% Methyl-2,4-pentanediol (MPD)., VAPOR DIFFUSION, SITTING DROP, temperature 293K |