4HT8
Crystal structure of E coli Hfq bound to poly(A) A7
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | SSRF BEAMLINE BL17U |
| Synchrotron site | SSRF |
| Beamline | BL17U |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2010-02-04 |
| Detector | MARMOSAIC 225 mm CCD |
| Wavelength(s) | 0.9793 |
| Spacegroup name | C 1 2 1 |
| Unit cell lengths | 103.990, 40.710, 100.850 |
| Unit cell angles | 90.00, 101.36, 90.00 |
Refinement procedure
| Resolution | 98.870 - 1.900 |
| R-factor | 0.1917 |
| Rwork | 0.190 |
| R-free | 0.23140 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 1hk9 |
| RMSD bond length | 0.012 |
| RMSD bond angle | 1.345 |
| Data reduction software | MOSFLM |
| Data scaling software | SCALA (3.3.15) |
| Phasing software | PHASER (2.1.4) |
| Refinement software | REFMAC |
Data quality characteristics
| Overall | Inner shell | Outer shell | |
| Low resolution limit [Å] | 98.874 | 31.150 | 1.790 |
| High resolution limit [Å] | 1.700 | 5.380 | 1.700 |
| Rmerge | 0.037 | 0.214 | |
| Total number of observations | 4312 | 24392 | |
| Number of reflections | 45603 | ||
| <I/σ(I)> | 12.1 | 15.3 | 3.5 |
| Completeness [%] | 99.2 | 83.3 | 100 |
| Redundancy | 3.6 | 3.4 | 3.7 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 7.9 | 283 | 200 mM NH4Ac, 100 Tris, 26% 2-propanol, pH 7.9, VAPOR DIFFUSION, HANGING DROP, temperature 283K |
