4HSP

Crystal structure of a DUF3299 family protein (PA4066) from Pseudomonas aeruginosa PAO1 at 2.45 A resolution

Experimental procedure

Experimental method	MAD
Source type	SYNCHROTRON
Source details	ALS BEAMLINE 8.2.2
Synchrotron site	ALS
Beamline	8.2.2
Temperature [K]	100
Detector technology	CCD
Collection date	2012-09-20
Detector	ADSC Q315
Wavelength(s)	0.979111,0.979338,0.918401
Spacegroup name	P 62 2 2
Unit cell lengths	119.033, 119.033, 60.972
Unit cell angles	90.00, 90.00, 120.00

Refinement procedure

Resolution	29.234 - 2.450
R-factor	0.1749
Rwork	0.173
R-free	0.21300
Structure solution method	MAD
RMSD bond length	0.010
RMSD bond angle	1.190
Data reduction software	XDS
Data scaling software	XSCALE (March 15, 2012)
Phasing software	SHELX
Refinement software	BUSTER-TNT (2.10.0)

Data quality characteristics

	Overall	Inner shell	Outer shell
Low resolution limit [Å]	29.234		2.540
High resolution limit [Å]	2.450	5.250	2.450
Rmerge	0.086	0.014	0.014
Number of reflections	9810	1826	1784
<I/σ(I)>	17.37	43.1	1.6
Completeness [%]	99.4	99.2	96.3

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, SITTING DROP	6.5	277	0.2M sodium chloride, 2.0M ammonium sulfate, 0.1M sodium cacodylate pH 6.5, NANODROP, VAPOR DIFFUSION, SITTING DROP, temperature 277K