4HSE
Crystal structure of ClpB NBD1 in complex with guanidinium chloride and ADP
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | SLS BEAMLINE X10SA |
| Synchrotron site | SLS |
| Beamline | X10SA |
| Temperature [K] | 100 |
| Detector technology | PIXEL |
| Collection date | 2012-06-17 |
| Detector | PSI PILATUS 6M |
| Wavelength(s) | 0.9785 |
| Spacegroup name | P 31 2 1 |
| Unit cell lengths | 60.990, 60.990, 213.870 |
| Unit cell angles | 90.00, 90.00, 120.00 |
Refinement procedure
| Resolution | 47.360 - 2.200 |
| R-factor | 0.22742 |
| Rwork | 0.225 |
| R-free | 0.26631 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 1QVR residues 141-534 |
| RMSD bond length | 0.009 |
| RMSD bond angle | 1.189 |
| Data reduction software | XDS |
| Data scaling software | XDS |
| Phasing software | PHASER |
| Refinement software | REFMAC (5.5.0102) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 50.000 | 2.300 |
| High resolution limit [Å] | 2.200 | 2.200 |
| Rmerge | 0.054 | 0.391 |
| Number of reflections | 24236 | |
| <I/σ(I)> | 19.5 | 4.8 |
| Completeness [%] | 99.4 | 99.1 |
| Redundancy | 4.9 | 5.2 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 7.5 | 293 | 0.1 M Tris/HCl pH 7.5, 1.0 M LiCl, 18 (w/v) % PEG 6000, 10 mM MgCl2, 10 mM GdmCl, VAPOR DIFFUSION, HANGING DROP, temperature 293K |
