4HR1
Structure of PAV1-137, a protein from the virus PAV1 that infects Pyrococcus abyssi.
Experimental procedure
| Experimental method | SAD |
| Source type | SYNCHROTRON |
| Source details | ESRF BEAMLINE ID14-4 |
| Synchrotron site | ESRF |
| Beamline | ID14-4 |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2006-10-20 |
| Detector | ADSC QUANTUM 315r |
| Wavelength(s) | 0.9793 |
| Spacegroup name | P 61 2 2 |
| Unit cell lengths | 73.546, 73.546, 191.549 |
| Unit cell angles | 90.00, 90.00, 120.00 |
Refinement procedure
| Resolution | 19.220 - 2.500 |
| R-factor | 0.2343 |
| Rwork | 0.232 |
| R-free | 0.28030 |
| Structure solution method | SAD |
| RMSD bond length | 0.010 |
| RMSD bond angle | 1.130 |
| Data reduction software | MOSFLM |
| Data scaling software | SCALA |
| Phasing software | SHARP |
| Refinement software | BUSTER (2.8.0) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 20.000 | 2.320 |
| High resolution limit [Å] | 2.200 | 2.200 |
| Rmerge | 0.058 | 0.459 |
| Number of reflections | 15911 | |
| <I/σ(I)> | 19 | 2.4 |
| Completeness [%] | 97.4 | 85.3 |
| Redundancy | 5.9 | 2.8 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, SITTING DROP | 4 | 291 | 35% PEG400, 0.5M NH4Cl, 0.1M Na citrate, VAPOR DIFFUSION, SITTING DROP, temperature 291K |
