4HQD
Crystal Structure of Engineered Protein. Northeast Structural Genomics Consortium Target OR265.
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | NSLS BEAMLINE X4A |
Synchrotron site | NSLS |
Beamline | X4A |
Temperature [K] | 100 |
Detector technology | CCD |
Collection date | 2012-10-11 |
Detector | ADSC QUANTUM 4 |
Wavelength(s) | 0.97905 |
Spacegroup name | P 1 21 1 |
Unit cell lengths | 69.004, 30.461, 70.061 |
Unit cell angles | 90.00, 92.68, 90.00 |
Refinement procedure
Resolution | 34.992 - 2.433 |
R-factor | 0.199 |
Rwork | 0.197 |
R-free | 0.22700 |
Structure solution method | MIR |
Starting model (for MR) | 4hb5 |
RMSD bond length | 0.006 |
RMSD bond angle | 1.169 |
Data reduction software | HKL-2000 |
Data scaling software | HKL-2000 |
Phasing software | BALBES |
Refinement software | PHENIX (1.7.2_869) |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 50.000 | 2.520 |
High resolution limit [Å] | 2.430 | 2.430 |
Rmerge | 0.094 | 0.212 |
Number of reflections | 20809 | |
<I/σ(I)> | 17.6 | 4.34 |
Completeness [%] | 97.9 | 95.1 |
Redundancy | 2.4 | 2.3 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | Microbatch crystallization under oil | 7.5 | 277 | 40% PEG 400, 0.1M lithium sulfate, 0.1M HEPES, pH 7.5, Microbatch crystallization under oil, temperature 277K |