4HPY

Crystal structure of RV144-elicited antibody CH59 in complex with V2 peptide

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	APS BEAMLINE 22-BM
Synchrotron site	APS
Beamline	22-BM
Temperature [K]	200
Detector technology	CCD
Collection date	2012-04-12
Detector	MARMOSAIC 225 mm CCD
Wavelength(s)	1.00
Spacegroup name	P 21 21 21
Unit cell lengths	41.888, 79.150, 127.112
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	19.892 - 1.500
R-factor	0.1715
Rwork	0.171
R-free	0.18730
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	4hpo
RMSD bond length	0.004
RMSD bond angle	0.998
Data reduction software	HKL-2000
Data scaling software	HKL-2000
Phasing software	PHASER
Refinement software	PHENIX ((phenix.refine: 1.8.1_1168))

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	20.000	1.530
High resolution limit [Å]	1.500	1.500
Rmerge		0.874
Number of reflections	68734
Completeness [%]	100.0	100
Redundancy		5.6

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, HANGING DROP	6.5	293	15% (w/v) PEG8000, 30% isopropanol, 0.1M imidazole pH 6.5, VAPOR DIFFUSION, HANGING DROP, temperature 293K