4HP9
Crystal structure of the N-terminal truncated PAS domain from the hERG potassium channel
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | SLS BEAMLINE X06DA |
| Synchrotron site | SLS |
| Beamline | X06DA |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2010-10-18 |
| Detector | MARCCD |
| Wavelength(s) | 1.00150 |
| Spacegroup name | P 42 3 2 |
| Unit cell lengths | 89.528, 89.528, 89.528 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 44.764 - 2.120 |
| R-factor | 0.1959 |
| Rwork | 0.194 |
| R-free | 0.23800 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 1byw |
| RMSD bond length | 0.008 |
| RMSD bond angle | 1.033 |
| Data reduction software | MOSFLM |
| Data scaling software | SCALA (3.3.9) |
| Phasing software | PHASER |
| Refinement software | PHENIX (1.6.4_486) |
Data quality characteristics
| Overall | Inner shell | Outer shell | |
| Low resolution limit [Å] | 89.528 | 89.528 | 2.230 |
| High resolution limit [Å] | 2.120 | 6.700 | 2.120 |
| Rmerge | 0.021 | 0.691 | |
| Rmeas | 0.023 | 0.783 | |
| Rpim | 0.008 | 0.270 | |
| Total number of observations | 2417 | 7979 | |
| Number of reflections | 7429 | ||
| <I/σ(I)> | 21.7 | 62 | 2.9 |
| Completeness [%] | 99.5 | 100 | 97.1 |
| Redundancy | 9.9 | 8 | 7.8 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, SITTING DROP | 298 | 30% PEG-MME2000, 0.1M potasiumm thiocyanate, 1.0% benzamidine-HCl, vapor diffusion, sitting drop, temperature 298K |
