4HNZ
Crystal structure of eukaryotic HslV from Trypanosoma brucei
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | SPRING-8 BEAMLINE BL44XU |
| Synchrotron site | SPring-8 |
| Beamline | BL44XU |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2011-07-02 |
| Detector | BRUKER SMART 6500 |
| Spacegroup name | P 1 21 1 |
| Unit cell lengths | 100.881, 106.990, 132.656 |
| Unit cell angles | 90.00, 104.24, 90.00 |
Refinement procedure
| Resolution | 41.130 - 2.393 |
| R-factor | 0.21905 |
| Rwork | 0.218 |
| R-free | 0.23866 |
| Structure solution method | MOLECULAR REPLACEMENT |
| RMSD bond length | 0.016 |
| RMSD bond angle | 1.646 |
| Data reduction software | HKL-2000 |
| Data scaling software | HKL-2000 |
| Phasing software | MOLREP |
| Refinement software | REFMAC (5.7.0029) |
Data quality characteristics
| Overall | |
| Low resolution limit [Å] | 50.000 |
| High resolution limit [Å] | 2.393 |
| Number of reflections | 205404 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, SITTING DROP | 5.5 | 293 | 0.1M Acetate pH 5.5, 2.0M Ammonium sulfate, 2% PEG 400 , VAPOR DIFFUSION, SITTING DROP, temperature 293K |
