4HNQ

Crystal Structure of the mutant Q97A of Vibrio cholerae CheY3

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	ROTATING ANODE
Source details	RIGAKU RU200
Temperature [K]	100
Detector technology	IMAGE PLATE
Collection date	2012-02-17
Detector	MAR scanner 345 mm plate
Spacegroup name	H 3
Unit cell lengths	67.640, 67.640, 74.918
Unit cell angles	90.00, 90.00, 120.00

Refinement procedure

Resolution	23.070 - 2.400
R-factor	0.225
Rwork	0.225
R-free	0.25200
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	3to5
RMSD bond length	0.014
RMSD bond angle	1.900
Data reduction software	AUTOMAR
Phasing software	AMoRE
Refinement software	CNS (1.2)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	23.070
High resolution limit [Å]	2.400	2.400
Number of reflections	4141
Completeness [%]	85.5

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, HANGING DROP	8	293	1.6 M ammonium sulfate, 0.1 M Tris, pH 8.0, VAPOR DIFFUSION, HANGING DROP, temperature 293K