4HNO
High resolution crystal structure of DNA Apurinic/apyrimidinic (AP) endonuclease IV Nfo from Thermatoga maritima
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | SSRL BEAMLINE BL9-1 |
Synchrotron site | SSRL |
Beamline | BL9-1 |
Temperature [K] | 90 |
Detector technology | IMAGE PLATE |
Collection date | 1999-07-15 |
Detector | MAR scanner 345 mm plate |
Wavelength(s) | 0.78 |
Spacegroup name | P 61 |
Unit cell lengths | 123.370, 123.370, 35.395 |
Unit cell angles | 90.00, 90.00, 120.00 |
Refinement procedure
Resolution | 24.511 - 0.919 |
R-factor | 0.1255 |
Rwork | 0.125 |
R-free | 0.13900 |
Structure solution method | MOLECULAR REPLACEMENT |
Starting model (for MR) | 1qtw |
RMSD bond length | 0.017 |
RMSD bond angle | 1.311 |
Data reduction software | DENZO |
Data scaling software | SCALEPACK |
Phasing software | EPMR (2.1) |
Refinement software | PHENIX (1.8.1_1168) |
Data quality characteristics
Overall | Inner shell | Outer shell | |
Low resolution limit [Å] | 30.000 | 30.000 | 0.950 |
High resolution limit [Å] | 0.919 | 2.110 | 0.919 |
Rmerge | 0.044 | 0.420 | |
Number of reflections | 204938 | ||
<I/σ(I)> | 10.8 | ||
Completeness [%] | 95.9 | 96.6 | 88.6 |
Redundancy | 2.84 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, HANGING DROP | 9 | 298 | 100 mM Tris-HCl pH 9.0, 4 mM DTT, 1.5% saturated MgSO4, 16% ethylene glycol, 20% mPEG2K, VAPOR DIFFUSION, HANGING DROP, temperature 298K |