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4HNO

High resolution crystal structure of DNA Apurinic/apyrimidinic (AP) endonuclease IV Nfo from Thermatoga maritima

Experimental procedure
Experimental methodSINGLE WAVELENGTH
Source typeSYNCHROTRON
Source detailsSSRL BEAMLINE BL9-1
Synchrotron siteSSRL
BeamlineBL9-1
Temperature [K]90
Detector technologyIMAGE PLATE
Collection date1999-07-15
DetectorMAR scanner 345 mm plate
Wavelength(s)0.78
Spacegroup nameP 61
Unit cell lengths123.370, 123.370, 35.395
Unit cell angles90.00, 90.00, 120.00
Refinement procedure
Resolution24.511 - 0.919
R-factor0.1255
Rwork0.125
R-free0.13900
Structure solution methodMOLECULAR REPLACEMENT
Starting model (for MR)1qtw
RMSD bond length0.017
RMSD bond angle1.311
Data reduction softwareDENZO
Data scaling softwareSCALEPACK
Phasing softwareEPMR (2.1)
Refinement softwarePHENIX (1.8.1_1168)
Data quality characteristics
 OverallInner shellOuter shell
Low resolution limit [Å]30.00030.0000.950
High resolution limit [Å]0.9192.1100.919
Rmerge0.0440.420
Number of reflections204938
<I/σ(I)>10.8
Completeness [%]95.996.688.6
Redundancy2.84
Crystallization Conditions
crystal IDmethodpHtemperaturedetails
1VAPOR DIFFUSION, HANGING DROP9298100 mM Tris-HCl pH 9.0, 4 mM DTT, 1.5% saturated MgSO4, 16% ethylene glycol, 20% mPEG2K, VAPOR DIFFUSION, HANGING DROP, temperature 298K

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