4HN9
Crystal structure of iron ABC transporter solute-binding protein from Eubacterium eligens
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | APS BEAMLINE 19-ID |
| Synchrotron site | APS |
| Beamline | 19-ID |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2012-10-10 |
| Detector | ADSC QUANTUM 315r |
| Wavelength(s) | 0.97923 |
| Spacegroup name | P 1 21 1 |
| Unit cell lengths | 61.870, 57.564, 79.299 |
| Unit cell angles | 90.00, 98.20, 90.00 |
Refinement procedure
| Resolution | 30.012 - 1.850 |
| R-factor | 0.1509 |
| Rwork | 0.150 |
| R-free | 0.19820 |
| Structure solution method | SAD |
| RMSD bond length | 0.014 |
| RMSD bond angle | 1.395 |
| Data reduction software | HKL-3000 |
| Data scaling software | HKL-3000 |
| Phasing software | SHELXD |
| Refinement software | PHENIX ((phenix.refine: 1.8_1069)) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 30.000 | 1.880 |
| High resolution limit [Å] | 1.850 | 1.850 |
| Rmerge | 0.084 | 0.724 |
| Number of reflections | 47253 | |
| <I/σ(I)> | 13.8 | 1.98 |
| Completeness [%] | 99.9 | 100 |
| Redundancy | 3.7 | 3.7 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, SITTING DROP | 8 | 297 | 35% PEG4000, pH 8.0, VAPOR DIFFUSION, SITTING DROP, temperature 297K |
