4HMZ
Crystal Structure of ChmJ, a 3'-monoepimerase from Streptomyces bikiniensis in complex with dTDP-quinovose
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | ROTATING ANODE |
| Source details | RIGAKU RU200 |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2012-04-10 |
| Detector | Bruker Platinum 135 |
| Wavelength(s) | 1.5418 |
| Spacegroup name | I 4 |
| Unit cell lengths | 140.721, 140.721, 117.736 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 30.000 - 2.000 |
| R-factor | 0.20165 |
| Rwork | 0.199 |
| R-free | 0.24883 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 2c0z |
| RMSD bond length | 0.009 |
| RMSD bond angle | 2.217 |
| Data reduction software | SAINT |
| Data scaling software | SADABS |
| Phasing software | PHASER |
| Refinement software | REFMAC (5.5.0109) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 99.500 | 2.100 |
| High resolution limit [Å] | 2.000 | 2.000 |
| Rmerge | 0.088 | |
| Number of reflections | 73347 | |
| <I/σ(I)> | 1.95 | |
| Completeness [%] | 94.9 | 89.2 |
| Redundancy | 4.2 | 1.78 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 7 | 298 | 20% PEG 3400, 2% 2-methyl-2,4-pentanediol, 100 mM MOPS, pH 7.0, VAPOR DIFFUSION, HANGING DROP, temperature 298K |
