4HMT

Crystal structure of PhzG from Pseudomonas fluorescens 2-79 in complex with hexahydrophenazine-1,6-dicarboxylic acid

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	SLS BEAMLINE X10SA
Synchrotron site	SLS
Beamline	X10SA
Temperature [K]	100
Detector technology	CCD
Collection date	2009-02-16
Detector	MARMOSAIC 225 mm CCD
Wavelength(s)	0.98004
Spacegroup name	P 21 21 21
Unit cell lengths	57.593, 63.425, 133.546
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	43.612 - 1.420
R-factor	0.1175
Rwork	0.116
R-free	0.15300
Structure solution method	FOURIER SYNTHESIS
RMSD bond length	0.017
RMSD bond angle	1.667
Data scaling software	XSCALE
Refinement software	PHENIX (1.8.1_1168)

Data quality characteristics

	Overall
Low resolution limit [Å]	45.000
High resolution limit [Å]	1.420
Number of reflections	89891

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, HANGING DROP		298	21-24% (w/v) PEG 3350, 0.2M (NH4)2SO4, 0.1M BisTris, 15uM PhzF, 30uM PhzA/B, 0.2M TRIS-HCl pH 7.5, vapor diffusion, hanging drop, temperature 298K