4HMR
Crystal structure of mutant rabbit PRP 121-230 (S170N/S174N)
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | CLSI BEAMLINE 08ID-1 |
Synchrotron site | CLSI |
Beamline | 08ID-1 |
Temperature [K] | 100 |
Detector technology | CCD |
Collection date | 2008-07-08 |
Detector | MARMOSAIC 300 mm CCD |
Wavelength(s) | 0.9793 |
Spacegroup name | P 21 21 21 |
Unit cell lengths | 29.530, 86.440, 87.090 |
Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
Resolution | 61.350 - 1.600 |
R-factor | 0.15762 |
Rwork | 0.155 |
R-free | 0.20713 |
Structure solution method | MOLECULAR REPLACEMENT |
Starting model (for MR) | 3o79 |
RMSD bond length | 0.013 |
RMSD bond angle | 1.505 |
Data reduction software | XDS |
Data scaling software | XSCALE |
Phasing software | PHASER |
Refinement software | REFMAC (5.7.0029) |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 60.000 | 1.700 |
High resolution limit [Å] | 1.600 | 1.600 |
Rmerge | 0.079 | 0.372 |
Number of reflections | 30243 | |
<I/σ(I)> | 23.01 | 5.34 |
Completeness [%] | 99.7 | 100 |
Redundancy | 7.16 | 7.3 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, HANGING DROP | 6.5 | 298 | 100mM SODIUM CACODYLATE PH 6.5, 3.0M NACL, 30% GLYCEROL (cryoprotectant), VAPOR DIFFUSION, HANGING DROP, temperature 298K |