4HMI
Crystal structure of Staphylococcal nuclease variant Delta+PHS V99K at cryogenic temperature
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SEALED TUBE |
| Source details | OTHER |
| Temperature [K] | 100 |
| Detector technology | FLAT PANEL |
| Collection date | 2012-03-17 |
| Detector | PHOTON 100 |
| Wavelength(s) | 1.54 |
| Spacegroup name | P 1 21 1 |
| Unit cell lengths | 31.161, 61.124, 38.115 |
| Unit cell angles | 90.00, 92.95, 90.00 |
Refinement procedure
| Resolution | 19.220 - 1.750 |
| R-factor | 0.1899 |
| Rwork | 0.188 |
| R-free | 0.22090 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 3bdc |
| RMSD bond length | 0.019 |
| RMSD bond angle | 1.853 |
| Data reduction software | APEX |
| Phasing software | PHASER (2.2.1) |
| Refinement software | REFMAC |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 19.220 | 1.850 |
| High resolution limit [Å] | 1.750 | 1.750 |
| Rmerge | 0.031 | 0.243 |
| Number of reflections | 14469 | |
| <I/σ(I)> | 19.14 | 3.7 |
| Completeness [%] | 99.8 | 100 |
| Redundancy | 6.86 | 3.5 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 8 | 277 | 20% MPD, 25 mM potassium phosphate, with calcium chloride and pdTp added to protein in a 3:2:1 ratio, pH 8, VAPOR DIFFUSION, HANGING DROP, temperature 277K |
