4HKU
The crystal structure of TetR transcriptional regulator (lmo2814) from Listeria monocytogenes EGD-e
Experimental procedure
Experimental method | MAD |
Source type | SYNCHROTRON |
Source details | APS BEAMLINE 19-ID |
Synchrotron site | APS |
Beamline | 19-ID |
Temperature [K] | 100 |
Detector technology | CCD |
Collection date | 2012-08-22 |
Detector | ADSC QUANTUM 315r |
Wavelength(s) | 0.97926, 0.97951 |
Spacegroup name | P 21 21 21 |
Unit cell lengths | 46.668, 81.560, 105.425 |
Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
Resolution | 30.708 - 2.302 |
R-factor | 0.1976 |
Rwork | 0.195 |
R-free | 0.25670 |
Structure solution method | MAD |
RMSD bond length | 0.008 |
RMSD bond angle | 0.999 |
Data reduction software | HKL-3000 |
Data scaling software | HKL-3000 |
Phasing software | SHELXD |
Refinement software | PHENIX ((phenix.refine: 1.7.1_743)) |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 31.000 | 2.340 |
High resolution limit [Å] | 2.300 | 2.300 |
Rmerge | 0.102 | 0.740 |
Number of reflections | 18258 | |
<I/σ(I)> | 26.4 | 2.4 |
Completeness [%] | 98.9 | 98.1 |
Redundancy | 7.1 | 6.9 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, SITTING DROP | 7.5 | 289 | 0.2M Sodium Acetate, 0.1M HEPES:NaOH, 20%(w/v) PEG3000, pH 7.5, VAPOR DIFFUSION, SITTING DROP, temperature 289K |