4HJ5

Crystal Structure of Schizosaccharomyces pombe Pot1pC bound to ssDNA (GGTTTCGGT)

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	ROTATING ANODE
Source details	RIGAKU RUH2R
Temperature [K]	100
Detector technology	IMAGE PLATE
Collection date	2012-02-07
Detector	RIGAKU RAXIS IV++
Wavelength(s)	1.54
Spacegroup name	P 21 21 21
Unit cell lengths	40.873, 59.244, 65.763
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	29.951 - 2.040
R-factor	0.2295
Rwork	0.226
R-free	0.26090
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	4hik
RMSD bond length	0.008
RMSD bond angle	1.097
Data reduction software	DENZO
Data scaling software	SCALEPACK
Phasing software	PHENIX (1.7_650)
Refinement software	PHENIX (1.7_650)

Data quality characteristics

	Overall	Inner shell	Outer shell
Low resolution limit [Å]	50.000	50.000	2.120
High resolution limit [Å]	2.040	4.420	2.050
Rmerge	0.077	0.045	0.411
Number of reflections	9855
<I/σ(I)>	10.6
Completeness [%]	93.0	89.1	95
Redundancy	2.4	2.2	2.5

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, HANGING DROP	7	290	35% PEG 6000, 0.2M sodium formate, pH 7, VAPOR DIFFUSION, HANGING DROP, temperature 290K