4HJ1
Crystal structure of glycoprotein C from Rift Valley Fever Virus (glycosylated)
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | APS BEAMLINE 24-ID-C |
| Synchrotron site | APS |
| Beamline | 24-ID-C |
| Temperature [K] | 100 |
| Detector technology | PIXEL |
| Collection date | 2012-07-18 |
| Detector | PSI PILATUS 6M |
| Wavelength(s) | 0.9795 |
| Spacegroup name | P 1 21 1 |
| Unit cell lengths | 128.680, 56.370, 140.190 |
| Unit cell angles | 90.00, 96.55, 90.00 |
Refinement procedure
| Resolution | 29.760 - 1.900 |
| R-factor | 0.21606 |
| Rwork | 0.214 |
| R-free | 0.25464 |
| Structure solution method | SAD |
| RMSD bond length | 0.009 |
| RMSD bond angle | 1.508 |
| Data reduction software | XDC |
| Data scaling software | XSCALE |
| Phasing software | SOLVE |
| Refinement software | REFMAC (5.6.0117) |
Data quality characteristics
| Overall | Inner shell | Outer shell | |
| Low resolution limit [Å] | 139.270 | 25.000 | 1.950 |
| High resolution limit [Å] | 1.900 | 20.000 | 1.900 |
| Rmerge | 0.072 | 0.010 | 0.010 |
| Number of reflections | 154051 | ||
| <I/σ(I)> | 10.3 | 30.32 | 1.4 |
| Completeness [%] | 97.4 | 97.4 | 85.6 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 6.2 | 289 | 18% PEG 5000 MME, 0.1 M MES, O.1 M Ammonium sulfate, 5% glycerol, 1.8 mM UDM, pH 6.2, vapor diffusion, hanging drop, temperature 289K |
