4HIK
Crystal Structure of Schizosaccharomyces pombe Pot1pC bound to ssDNA (GGTTACGGT)
Experimental procedure
| Experimental method | MAD |
| Source type | SYNCHROTRON |
| Source details | ALS BEAMLINE 8.2.1 |
| Synchrotron site | ALS |
| Beamline | 8.2.1 |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2011-10-05 |
| Detector | ADSC QUANTUM 315r |
| Wavelength(s) | 0.9799, 0.9801, 0.9428 |
| Spacegroup name | P 21 21 21 |
| Unit cell lengths | 44.870, 57.390, 66.170 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 35.348 - 1.636 |
| R-factor | 0.1947 |
| Rwork | 0.194 |
| R-free | 0.21570 |
| Structure solution method | MAD |
| RMSD bond length | 0.006 |
| RMSD bond angle | 1.058 |
| Data reduction software | DENZO |
| Data scaling software | SCALEPACK |
| Phasing software | SnB |
| Refinement software | PHENIX (1.7_650) |
Data quality characteristics
| Overall | Inner shell | Outer shell | |
| Low resolution limit [Å] | 50.000 | 50.000 | 1.670 |
| High resolution limit [Å] | 1.636 | 4.450 | 1.640 |
| Rmerge | 0.041 | 0.024 | 0.134 |
| <I/σ(I)> | 26.4 | ||
| Completeness [%] | 99.1 | 98.5 | 93.8 |
| Redundancy | 4.5 | 4.6 | 3.6 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 7 | 290 | 25% w/V PEG 4000, 0.2M ammonium formate, pH 7, VAPOR DIFFUSION, HANGING DROP, temperature 290K |
