4HI5

crystal structure of F37A mutant of borna disease virus matrix protein

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	BESSY BEAMLINE 14.1
Synchrotron site	BESSY
Beamline	14.1
Temperature [K]	100
Detector technology	CCD
Collection date	2010-07-10
Detector	MARMOSAIC 225 mm CCD
Spacegroup name	I 4 3 2
Unit cell lengths	139.637, 139.637, 139.637
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	70.000 - 3.600
R-factor	0.2477
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	3f1j
RMSD bond length	0.006
RMSD bond angle	1.008
Data reduction software	XDS
Data scaling software	SCALA
Phasing software	MOLREP
Refinement software	REFMAC (5.5.0109)

Data quality characteristics

	Overall
Low resolution limit [Å]	70.000
High resolution limit [Å]	3.600
Rmerge	0.134
Number of reflections	2918
<I/σ(I)>	13.4
Completeness [%]	100.0
Redundancy	11

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, HANGING DROP	7.1	293	0.2 M MgCl2, 30% w/v PEG400, 0.1 M HEPES, pH 7.1, VAPOR DIFFUSION, HANGING DROP, temperature 293K